Acute electronic vapour product whole aerosol exposure of 3D human bronchial tissue results in minimal cellular and transcriptomic responses when compared to cigarette smoke

The use of electronic vapour products (EVPs) continues to increase worldwide and with advances in cell culture systems, molecular biology the computational sciences there is also accumulating evidence their potential reduced toxicity harm when compared cigarette smoke. To further understand risks health effects associated exposure EVP aerosols we have assessed cellular transcriptomic response from a commercially available lung tissue system (MucilAir TM ) following single sub-cytotoxic smoke equivalent nicotine delivered dose aerosol. transcriptomic, (cilia beat frequency (CBF) percent active area (%AA), trans epithelial electrical resistance (TEER), histology) cytokine release were at 4- 48- hours recovery air, aerosol (8.4% V/V: myblu blueberry flavour, 2.4% nicotine) 3R4F (3.5% exposure). No pathological changes observed either time point any exposure. Air had no effect on CBF, %AA nor TEER 48 hours. Exposure resulted decrease TEER, an CBF proinflammatory cytokines both points. Although number significantly expressed genes was minimal aerosol, significant upregulation several disease relevant pathways. These data provide that acute less damage cells than equivalent, based,